Spectrophotomètre UV-Visible RB-10 livré avec passeur manuel 4 cellules

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Spécifications techniques

The Halo RB-10 is a ratio beam spectrophotometer with an extensive array of built-in functions for versatility and suitability to many analytical and biological applications. The ratio beam optics impart greater accuracy and reproducibility than the conventional, single beam optics.

Ratio Beam Optics

The absorbance signal in conventional single beam instruments can destabilize and result in data inaccuracy. By contrast, ratio beam optics compensate and stabilize signal fluctuations to increase accuracy and reliability even over prolonged usage such as in time course and kinetic measurements. The ratio beam principle involves splitting the beam generated by the light source by a half mirror. One of the split beams passes through the sample and is quantified by a detector whereas the other split beam, which is representative of the absorbance signal, is measured by an independent detector to obtain a signal reference. The ratio of the values from both detectors is then calculated to detect and compensate for any aberration in the energy of the light source or a temporal change of the optical elements and produce highly stable photometric values.

Spectral Features

Boasting a 2 nm spectral bandpass the Halo RB-10 offers superior spectra and peak resolution.

Other specifications include an impressive wavelength accuracy of ±0.5nm, noise level 0.0005Abs (500nm) and stray light 0.05% (220nm NaI, 340nm NaNO2). 

Dual Lamp Advantage

By virtue of the halogen tungsten and deuterium lamps typically found in higher end analytical spectrophotometers the Halo RB-10 wavelength range is an impressive 190nm – 1,100nm. Lamp switching is automatic (by default at 340nm) and both lamps are long life.

User Friendly Operation and Information Rich LCD Display

The 94mm x 70mm, backlit LCD screen with adjustable brightness control is sufficiently large to display a large array of data even in a graphical format. Furthermore, single wavelength absorbance or transmittance data can be enhanced and enlarged using the unique zoom function. The seamless and chemical resistant keypad is designed for easy and quick selection of navigation and function features whilst protecting against any laboratory spills.

Validation Functions

To ensure optimum instrument performance, a self-diagnostic function incorporating a number of parameters is executed each time the Halo RB-10 is switched on. Furthermore, the Halo RB-10 is equipped with a GLP/GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can be all validated and the audit report printed.

Stand Alone or PC Operation

The Halo RB-10 is fully equipped and capable of executing all functions in stand alone mode. Simply connect a standard laser printer for direct printouts of data and graphs. For more advanced analyses and reporting, the simple slide of a switch places the Halo RB-10 under the direct control of the optional UV Detective software installed in computer with Windows® XP or Windows® 7 operating system. 

Diverse Range of Measurement Modes

Photometry Mode: Perform quantitative analyses in either absorbance or transmittance modes. Select from single wavelength, up to 6 multiple individual wavelengths, nucleic acid/protein A260/A280 ratios and set up calibration curves with up to 20 standards for concentration measurements.

Time Scan: Perform kinetic measurements for time periods ranging from 1 minute to >27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.

Wavelength Scan: Perform a full spectral scan from 190 to 1,100nm at any of 8 incremental and preset selectable scan speeds starting from a high resolution 10nm/minute up to a swift 3,600nm /minute. Data is displayed as either numerical values or a graphical spectrum. Furthermore, perform downstream processing of data, such as peak / valley search or smoothing, directly on board or with the optional Halo UV Detective software. 


Wavelength Range

190nm -1,100 nm

Spectral Bandwidth


Stray Light

0.05% (220nm NaI, 340nm NaNO2)

Wavelength Accuracy


Photometric Range

Absorbance: -3 to +3
%T: 0% to 300% Concentration: 0,000 to 9,999

Photometry Accuracy (measured with NIST 930D)

±0.002Abs (0~0.5Abs)

±0.004Abs (0.5~1.0Abs)

±0.008Abs (1.0~2.0Abs)


Photometry Repeatability (measured with NIST 930D)

±0.001Abs (0~0.5Abs)

±0.002Abs (0.5~1.0Abs)

±0.004Abs (1.0~2.0Abs)


Wavelength Scan Speed

10, 100, 200, 400, 800, 1,200, 2,400, 3,600 nm/minute

Baseline Stability

0.001 Abs/hr (500nm, after 2 hours)

Noise Level

0.0005 Abs (500nm)

Light Source

Tungsten-Halogen and Deuterium Lamps

Light Source Switching

Automatic switching at 340nm


Silicon Photodiode


Back-lit LCD 94(W) x 70(H) mm


370(W) x 550(D) x 265(H) mm

Net Weight


Gross Weight


Power Requirements

110 - 220 V selectable, 50/60Hz

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