Spectrophotomètre UV-Visible double faisceau DB-20

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Spécifications techniques

The Halo DB-20 is a high performance double beam spectrophotometer suitable for many analytical applications that a higher level of accuracy is required. 

Genuine Double Beam Optics 

True double beam optics ensure concurrent measurement of the sample and reference for improved stability, accuracy and reproducibility. The light beam is split in two using a half mirror so that one beam passes through the sample side whilst the other passes through the reference side. Both beams are then measured on individual detectors. The reference side beam also acts to stabilize photometric values in a similar manner to the ratio beam principle. 

Spectral Features 

Coma aberration elimination from the concave diffraction grating achieves a high resolution 1.5 nm (DB-20S : 1nm) spectral bandpass and certifies compliance of the Halo DB-20 to the stringent European Pharmacopoeia standards. 

Other specifications include an impressive wavelength accuracy of ±0.3nm, noise level 0.0003Abs (500nm) and stray light ≤0.05% (220nm NaI, 340nm NaNO2). 

Built-in and Diverse Range of Measurement Modes 

Photometry Mode: Perform quantitative analyses in either absorbance or transmittance modes. Select from single wavelength, up to 6 different individual wavelengths, nucleic acid/protein A260/A280 ratios and set up calibration curves with up to 20 standards for concentration measurements. 

Time Scan: Perform kinetic measurements for time periods ranging from 1 minute to >27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set. 

Wavelength scan: Perform a full spectral scan from 190 to 1,100nm at any of 8 incremental and preset selectable scan speeds starting from a high resolution 10nm/minute up to a swift 3,600nm /minute. Data is displayed as either numerical values or a graphical spectrum. Furthermore, perform downstream processing of data, such as peak / valley search or smoothing, directly on board or with the optional Halo UV Detective software. 

Dual Lamp Advantage 

By virtue of the long life, halogen tungsten and deuterium lamps, the Halo DB-20s wavelength range is an extensive 190nm – 1,100nm. Lamp switching is automatic and selectable from a wavelength range of 325nm to 370nm.

User Friendly Operation and Information Rich LCD Display 

The extra large 165mm x 122mm, backlit LCD screen with adjustable brightness control displays a large array of data also in graphical format. The seamless and chemical resistant keypad is designed for easy and quick selection of navigation and function features whilst protecting against any laboratory spills. Other unique features include the ‘GO TO WL’ short cut key to allow direct input of a new wavelength into an existing measurement. 

Validation Functions 

To ensure optimum instrument performance, self-diagnosis incorporating a number of parameters and wavelength calibration are automatically initiated upon start-up. Furthermore, the Halo DB-20 is equipped with a GLP/ GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can be all validated and the audit report printed. 

Stand Alone or PC Operation 

The Halo DB-20 is fully equipped and capable of executing all functions in stand alone mode. For more advanced control, analyses and reporting, the simple slide of a switch places the Halo DB-20 under the direct control of the optional UV Detective software installed in computer with Windows® XP or Windows®7 operating system. 

On-Board Data Storage 

Up to 20 operating programs and up to 10 sets of measurement data can be stored in the flash memory of the Halo DB-20.
Programs can easily be recalled, edited and deleted. Furthermore, when in stand alone mode, data (in text format) can be downloaded directly to an external memory stick via the USB port and transferred for further processing to any computer loaded with commercial spreadsheets (such as Microsoft® Excel) 

PC Control Operation DB-20R 

The Halo DB-20R shares the same specification of DB-20S with impressive 1nm bandwidth. DB-20R is PC control only, which the display and USB storage port are replaced by the UV Detective software come as standard. 






Concave diffraction grating / Double beam principle

Wavelength Range

190nm -1,100 nm

Spectral Bandwidth

1.5 nm

1.0 nm

Stray Light

(220nm NaI, 340nm NaNO

(220nm NaI, 340nm NaNO

Wavelength Accuracy


Photometric Range

Absorbance: -3 to +3 %T: 0% to 300%T Concentration: 0,000 to 9,999

Photometry Accuracy
(Measured with NIST 930D Filter)

±0.002Abs (0~0.5Abs)

±0.004Abs (0.5~1.0Abs)

±0.008Abs (1.0~2.0Abs)


Photometry Reproducibility (Measured with NIST 930D Filter)

±0.001Abs (0~0.5Abs)

±0.002Abs (0.5~1.0Abs)

±0.004Abs (1.0~2.0Abs)


Wavelength Scan Speed

10, 100, 200, 400, 800, 1,200, 2,400, 3,600 nm/minute

Baseline Stability

0.0003 Abs/hr (500nm, after 2 hours)

Noise Level

0.0003 Abs (500nm)

Light Source

Tungsten-Halogen and Deuterium Lamps

Light Source Switching

Automatic switching selectable from 325nm to 370nm


Silicon Photodiode


Back-lit LCD 165(W) x 122(H) mm



505(W) x 590(D) x 265(H) mm

Net Weight


Gross Weight


Power Requirements

110 - 220 V selectable, 50/60Hz

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